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Gamma-oryzanol, Physicochemical and Antioxidant Properties of Stabilized Rice Bran Oil from Dough and Mature Grain Stages

Gamma-oryzanol, Physicochemical and Antioxidant Properties of Stabilized Rice Bran Oil from Dough and Mature Grain Stages

Original Research ArticleOct 17, 2024Vol. 25 No. 1 (2025) 10.55003/cast.2024.260012

Abstract

Rice bran samples from dough and mature grain stages of rice were stabilized by heating in a hot air oven at 70ºC for 3h (or moisture <5.0%). Unstabilized and stabilized rice bran samples were subsequently pressed for oil extraction using a screw press machine. The γ-oryzanol, physicochemical, and antioxidant properties of both the unstabilized and stabilized rice bran oil (RBO) were determined. The fat content of fresh rice bran (unstabilized) from dough stage (19.19 %w.b.) was higher than that of the mature stage (13.22 %w.b.). Unstabilized RBO from mature stage (URBO-MS) had the lowest L* (35.70) and the highest a* (4.28) values. The γ-oryzanol content detected by UV-Vis spectophotometer was not significantly different among URBO-MS, stabilized RBO from the dough stage (SRBO-DS), and crude commercial RBO (CRBO). While the γ-oryzanol content of SRBO-DS detected by reversed-phase HPLC with diode array detector (DAD) was the highest, with a value of 1.00 g/100 g oil. The DPPH scavenging activity of SRBO-DS was the highest while that of CRBO was the lowest. The ABTS radical scavenging activity of unstabilized RBO from the dough stage (URBO-DS), SRBO-DS, and URBO-MS were not significantly different and were higher than that of stabilized RBO from the mature stage (SRBO-MS) and CRBO. The peroxide and free fatty acid (FFA) contents of SRBO-DS were the lowest with values of 1.19 meg/kg and 4.84% (as oleic acid), respectively. This finding suggests that stabalizing rice bran at the dough stage can increase the color values (as L* and b* values), γ-oryzanol, and DPPH scavenging activity, and decrease peroxide and FFA values of RBO.  Thus, stabilized RBO from the dough stage grain may be a functional food with antioxidant activity.

References

1
Alam, M. N., Bristi, N. J., & Rafiquzzaman, M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2), 143-152. https://doi.org/10.1016/j.jsps.2012.05.002
2
Amarasinghe, B. M. W. P. K., Kumarasiri, M. P. M., & Gangodavilage, N. C. (2009). Effect of method of stabilization on aqueous extraction of rice bran oil. Food and Bioproducts Processing, 87(2), 108-114. https://doi.org/10.1016/j.fbp.2008.08.002
3
Andriani, R., Subroto, T., Ishmayana, S., & Kurnia, D. (2022). Enhancement methods of antioxidant capacity in rice bran: A Review. Foods, 11(19). https://doi.org/10.3390/foods11192994
4
AOAC. (1990). Official Methods of Analysis (15th ed.). Washington DC: Association of Official Analytical Chemist.
5
AOAC. (2000). Official Methods of Analysis (17th ed.). Gaithersburg: The Association of Official Analytical Chemists.

Author Information

Boonyabhorn Duangsa

Faculty of Business Administration and Information Technology, Rajamangala University of Technology Isan , Khon Kaen, Thailand

Porntip Rodpon

Faculty of Business Administration, Rajamangala University of Technology Isan , Nakhon Ratchasima, Thailand

Chompoonuch Khongla

Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima, Thailand

Sumalee Musika

Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima, Thailand

Piyamaporn Thiamjite

Faculty of Business Administration, Rajamangala University of Technology Isan , Nakhon Ratchasima, Thailand

Patcharida Khumpumuang

Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima, Thailand

Usana Jangkloy

Faculty of Business Administration, Rajamangala University of Technology Isan , Nakhon Ratchasima, Thailand

Surawee ๋Jampatech

Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima, Thailand

Piyamaporn Thiamjite

Faculty of Business Administration, Rajamangala University of Technology Isan , Nakhon Ratchasima, Thailand

Apichai Sawisit

Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima, Thailand

About this Article

Current Journal

Vol. 25 No. 1 (2025)

Type of Manuscript

Original Research Article

Keywords

dough grain stage
.rice bran oil
antioxidant activity
γ-oryzanol
RP-HPLC

Published

17 October 2024

DOI

10.55003/cast.2024.260012

Current Journal

Journal Cover
Vol. 25 No. 1 (2025)

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