A simple, accurate, precise, sensitive and a highly selective spectrophotometric method was developed and validated for the estimation of Zidovudine in bulk and pharmaceutical dosage forms. The stock solutions were prepared as per procedure and were scanned at maximum absorbance of 266 nm. The linearity was found in the concentration range of 2-20 µg / mL. The Coefficient of determination (r2) was 0.999. The regression equation was found to be Y = 0.0435. C + 0.0205 and % RSD was found to be 0.630. The developed method was validated according to ICH guidelines and was found to be accurate and precise. The validation parameters are linearity, accuracy, precision, limit of detection, limit of quantitation, robustness and ruggedness. Thus the proposed method can be successfully applied for the estimation of Zidovudine in bulk and pharmaceutical dosage forms.
Keywords: Zidovudine, Validation, ICH guidelines, Spectrophotometric method
*Corresponding author: Mobile Phone: 08978304042
E-mail: chejetisharadha@gmail.com
Sharada*, C. H. ., Channabasavaraj, K. P. ., & Mani, T. T. . (2018). Development of a Spectrophotometric Method for the Quantitative Estimation of Zidovudine Concentration in Bulk and Pharmaceutical Dosage Forms. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 1-8.
