The Use of Agricultural Wastes as Substrates for Cell Growth and Carboxymethyl Cellulase (CMCASE) Production by Bacillus subtilis, Escherichia coli and Rhizobium sp.

Abstract

Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922 and Rhizobium sp. DASA23010 were examined for carboxymethyl cellulase (CMCase) production. CMCase produced from all strains was characterized as cell-bound enzyme. All strains could grow in medium containing CMC as a sole carbon source. The highest activities obtained from B. subtilis, E. coli and Rhizobium sp. were not significantly different. Comparing the growth in three agricultural wastes, maximum cell numbers were obtained from the media containing pineapple peel, vegetable residue and corncob,respectively. Corncob stimulated higher production of CMCase than other agricultural wastes.

KEYWORDS: Carboxymethyl cellulase, CMCase, Bacillus subtilis, Escherichia coli, Rhizobium, agricultural wastes

E-mail: neelawan@su.ac.th

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Citation Format

Sudto, A. ., Punyathiti, Y. ., & Pongsilp*, N. . (2018). The Use of Agricultural Wastes as Substrates for Cell Growth and Carboxymethyl Cellulase (CMCASE) Production by Bacillus subtilis, Escherichia coli and Rhizobium sp.. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 84-92.

References

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[2] Lynd, L. R., Weimer, P. J., van Zyl, W. H. and Pretorius, I. S. 2002. Microbial cellulose utilization: Fundamentals and biotechnology, Microbiology and Molecular Biology Reviews,66(3), 506–577.
[3] Zhou, S. and Ingram, L. O. 2000. Synergistic hydrolysis of carboxymethyl cellulose and acid-swollen cellulose by two endoglucanases (CelZ and CelY) from Erwinia chrysanthemi, Journal of Bacteriology, 182(20), 5676–5682.
[4] da Silva, R., Lago, E. S., Merheb, C. W., Macchione, M. M., Park, Y. K. and Gomes, E.2005. Production of xylanase and CMCase on solid fermentation in different residues by Thermoascus aurantiacus miehe, Brazilian Journal of Microbiology, 36(3), 235–241.
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Author Information

Atchara Sudto

Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand

Yaowapa Punyathiti

Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand

Neelawan Pongsilp*

Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand

About this Article

Journal

Vol. 8 No. 2 (2008)

Type of Manuscript

Original Research Article

Keywords

Carboxymethyl cellulase, CMCase, Bacillus subtilis, Escherichia coli, Rhizobium, agricultural wastes

Published

30 March 2018

The Use of Agricultural Wastes as Substrates for Cell Growth and Carboxymethyl Cellulase (CMCASE) Production by Bacillus Subtilis, Escherichia Coli and Rhizobium Sp.

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Author Information

Atchara Sudto

Yaowapa Punyathiti

Neelawan Pongsilp*

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Keywords

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