Antifungal metabolites from Trichoderma spp. isolates TISTR 3167, KMC 5, SRS 4 and SCP III were extracted from cell pellets by methanol and also from culture filtrates by three different solvents, i.e. hexane, ethyl acetate and n-butanol and tested for growth inhibition of A. flavus IMI 242684 using paper disc diffusion assay on potato dextrose agar. Ethyl acetate extracts from TISTR 3167, KMC 5 and SRS 4 were most active against A. flavus IMI 242684 and 50 mg/ml was the optimal concentration. When ethyl acetate extracts of the 3 isolates at 50 mg/ml were further applied to peanut grains, the growth and aflatoxin (B1 and B2) production by A. flavus IMI 242684 were inhibited during 21 days of storage at room temperature.
Keyword: Antifungal metabolites, Aspergillus flavus, Trichoderma spp., aflatoxins, crude extract, peanut grains
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Jantarach*, J. ., & Thanaboripat, D. . (2018). The Efficacy of Ethyl Acetate Extract of Trichoderma Culture Broth on Growth Inhibition and Aflatoxin Production by Aspergillus flavus IMI 242684. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 19-29.
