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The Effect of Exogenous Spermidine and Wood Vinegar on Growth and Physiology of Rice (Oryza sativa L.) cv. RD6 under Salt Stress

The Effect of Exogenous Spermidine and Wood Vinegar on Growth and Physiology of Rice (Oryza Sativa L.) Cv. RD6 Under Salt Stress

Original Research ArticleJun 4, 2019Vol. 19 No. 3 (2019)

Abstract

Rice cv. RD6 is one of the most important crops for Thailand particularly for people and farmers in the northeastern part of the country. Saline soil in the northeastern part of Thailand is an important problem that causes low yield of rice. Treatment of plants with some plant growth regulators (PGR) such as polyamine or bio-stimulants, for example wood vinegar (WV), can induce physiological response so that plants become more tolerant to abiotic stresses including salinity. The objective of this study was to investigate the effects of spermidine (Spd) and WV on increasing tolerance of rice seedlings in saline soil under greenhouse condition. Rice seedlings were grown for 30 days in pots containing 5 kg soil. The plants were then sprayed with distilled water (control), WV (1:500), Spd (0.05, 0.1 and 0.5 mM) and mixture between WV (1:500) and Spd (0.05, 0.1 and 0.5 mM) for 5 days and then exposed to salt stress (150 mM NaCl) for 14 days. The result indicated that salt stress decreased net photosynthesis rate, maximal quantum yield of PSII photochemistry (Fv/Fm), but increased membrane damage as indicated by increase in electrolyte leakage (EL). Under salt stress, spraying with WV (1:500) and 0.1 mM Spd (with or without WV) tended to increase shoot and root growth, respectively. Spraying with 0.05 mM Spd and 0.5 mM Spd significantly reduced EL and increased Fv/Fm, respectively. The most effective solutions to promote shoot growth, root growth, reduce membrane damage and improve photochemical function of PSII were WV (1:500), 0.1 mM Spd, 0.05 mM Spd and 0.5 mM Spd, respectively.

 

Keywords: spermidine; wood vinegar; salt stress; photosynthesis; photosystem II; electrolyte leakage; glutinous rice

*Corresponding author: Tel.: 094-3848530

                                           E-mail: anuchatbank@gmail.com

How to Cite

Srisompan*, A. ., & Theerakulpisut, P. . (2019). The Effect of Exogenous Spermidine and Wood Vinegar on Growth and Physiology of Rice (Oryza sativa L.) cv. RD6 under Salt Stress. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 289-296.

References

  • Topark-Ngam, B., 2006. Saline Soils in Northeast Thailand. 1st ed. Khon Kaen: Khon Kaen Printing Co., Ltd. [In Thai]
  • Kanawapee, N., Sanitchon, J., Lontom, W. and Threerakulpisut, P., 2012. Evaluation of salt tolerance at the seedling stage in rice genotypes by growth performance, ion accumulation, proline and chlorophyll content. Plant and Soil, 358, 235-249.
  • Land Development Department, 2016. Farmer's Guide to Northeastern Saline Management. Ministry of Agriculture and Cooperatives : Bangkok.
  • Estaji, A., Roosta, H.R., Rezaei, S.A., Hosseini, S.S. and Niknam, N., 2018. Morphological, physiological and phytochemical response of different Satureja hortensis L. accessions to salinity in a greenhouse experiment. Journal of Applied Research on Medicinal and Aromatic, 10, 25-33.
  • Munns, R.and Tester, M., 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681.

Author Information

Anuchat Srisompan*

Salt-tolerant Rice Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand

Piyada Theerakulpisut

Salt-tolerant Rice Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand

About this Article

Journal

Vol. 19 No. 3 (2019)

Type of Manuscript

Original Research Article

Keywords

spermidine; wood vinegar; salt stress; photosynthesis; photosystem II; electrolyte leakage; glutinous rice

Published

4 June 2019