Study on Gene Transformation and Expressed Sequence Tags of Chaetomium Globosum

In order to improve the bio-control fungus- Chaetomium globosum, and develop a new bio-fungicide, the resistance gene to benzimidazole fungicides was transformed into the fungus, and the expressed sequence tags (ESTs) of the fungus were sequenced. The results showed that the plasmid constructed in this study could improve the transformation rate significantly, (i.e. nearly 9 times higher than before). A cDNA library was constructed from the mycelium of the fungus and 3507 clones were randomly selected for sequencing. 1381 sequences were obtained, 868 of them are new genes. Analysis of the identified clones indicated sequence similarity to the genes encoding proteins such as enzymes, regulatory factors and the product related to plant disease bio-control.

Keywords: Chaetomium globosum, benzimidazole resistance, gene transformation, expressed sequence tags

Corresponding author: E-mail: yangq@hope.hit.edu.cn