Optimal Condition for Chromosomal Imbalances Analysis of Cancer Genome by Comparative Genomic Hybridization

Comparative Genomic Hybridization (CGH) is a technique for analysis of copy number changes throughout the genome based on the hybridization of differentially labeled tumor (test DNA) and normal DNA (reference DNA) to normal human metaphase chromosome (target DNA). In this report, we suggested some critical steps such as metaphase preparation and an average fragment length of DNA probe which obtains a uniform and intense hybridization signal. CGH technique will show gain and loss of DNA sequence regions in cancer genome. Those regions may harbor candidate genes such as oncogenes and tumor suppressor genes playing important roles in the initiation and progression of cancer.

Keywords: chromosome imbalances, comparative genomic hybridization, cancer genome

Corresponding author: E-mail: cast@kmitl.ac.th