Plant-based biogenic nanoparticle synthesis has appeared as a feasible alternative to conventional approaches to chemical synthesis. As a result, several environmentally benign methods for the quick production of silver nanoparticles have been published in recent years. The methods employ aqueous extracts of plant components like leaves, bark, and roots. In the present study, silver nanoparticles were synthesized from an aqueous leaf extract of S. auriculata. UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive x-ray analysis (EDAX) were employed to validate the synthesized nanoparticles. The UV analysis showed a peak range between 390-420 nm. FTIR showed the functional groups present in the silver nanoparticles (AgNPs). Moreover, the synthesized AgNPs were tested for their antimicrobial activity against both gram-negative and gram-positive bacterial strains. The antioxidant properties were studied with DPPH, hydrogen peroxide, and nitric oxide scavenging activity assays, and a cytotoxic assay was conducted against the Huh-7 cell line by a MTT study. The results showed that the synthesized silver nanoparticles possessed strong antibacterial, antioxidant, and cytotoxic activities against the Huh-7 cell line, indicating that the silver nanoparticles might be used in the pharmaceutical industry and for novel biological applications.
