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Improvement in Biomass Production of a Microalga Chlorella sp. S2 Using Starch Processing Wastewater

Improvement in Biomass Production of a Microalga Chlorella sp. S2 Using Starch Processing Wastewater

Original Research ArticleDec 14, 2020Vol. 21 No. 2 (2021)

Abstract

Microalgae are promising resources for high-quality dietary supplements, pharmaceutical and biofuel production. This study attempted to improve the biomass of a microalga by cultivation in starch processing wastewater. A microalga identified as Chlorella sp. S2 by morphological criterion was isolated from the facultative pond of a noodle making plant. It was able to grow in the starch processing wastewater without addition of nutrients. To increase the biomass productivity of Chlorella sp. S2, inorganic nitrogen sources (NaNO3, NH4Cl and KNO3) were added into the starch processing wastewater. The optimum nitrogen source was potassium nitrate at 7.5 mM of nitrogen, which increased the number of Chlorella sp. S2 up to 1.41´107 cells/ml and the specific growth rate was 0.351 d-1. Under sunlight, the microalga Chlorella sp. S2 also produced high biomass concentration (2.23±0.04 g/l). It means that microalgal cultivation using starch processing wastewater is a great process to produce biomass. In addition, it is able to reduce organic carbon in the wastewater and reduce cost.

 

Keywords: microalgae; Chlorella; cultivation; starch processing wastewater; biomass production

Corresponding author: Tel.: (+66) 866395551

                                          E-mail: siripornyos@hotmail.com

References

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Hodaifa, G., Sanchez, S., Martinez, E. and Orpez, R., 2013. Biomass production of Scenedesmus bliquus from mixtures of urban and olive-oil mill wastewaters used as culture medium. Applied Energy, 104, 345-352.
2
Lam, M.K. and Lee, K.T., 2013. Effect of carbon source towards the growth of Chlorella vulgaris for CO2 bio-mitigation and biodiesel production. International Journal of Greenhouse Gas Control, 14, 169-176.
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Takeshita, T., Ota, T., Yamazaki, T., Hirata, A., Zachleder, V. and Kawano, S., 2014. Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresource Technology, 158, 127-134.
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Li, X., Xu, H. and Wu, Q., 2007. Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors. Biotechnology and Bioengineering, 98, 764-771.
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Katiyar, R., Gurjar, B.R., Bharti, R.K., Kumar, A., Biswas, S. and Pruthi, V., 2017. Heterotrophic cultivation of microalgae in photobioreactor using low cost crude glycerol for enhanced biodiesel production. Renewable Energy, 113, 1359-1365.

Author Information

Wannakorn Kitcha

Division of Environmental Science, Faculty of Liberal Arts and Science, Sisaket Rajabhat University, Sisaket, Thailand

Wannakorn Kitcha

Division of Biology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok, Thailand

About this Article

Current Journal

Vol. 21 No. 2 (2021)

Type of Manuscript

Original Research Article

Keywords

microalgae; Chlorella; cultivation; starch processing wastewater; biomass production

Published

14 December 2020

DOI

Current Journal

Journal Cover
Vol. 21 No. 2 (2021)

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