Expressed Sequence Tags (EST)

Abstract

The focus on genome research has shifted from structural genomics to functional annotation. A rapidly growing area of functional research is the generation of expressed sequence tags (EST) in which a large number of randomly selected cDNA clones are partially sequenced. The generation of EST has proven to be a rapid and economical approach to the identification and characterization of expressed genes. Currently expressed sequence tag projects have accumulated over 15 million un-annotated files from more than 5318 cDNA libraries deposited in the public domain. Therefore, in this review we summarize the advantages of EST research, strategies for EST study, weaknesses of EST analysis and finally conclude with our work in EST research.

Keywords: EST, genome, bioinformatics

Corresponding author: E-mail: yangq@hope.hit.edu.cn

How to Cite

Citation Format

Peigang, L. ., & Qian*, Y. . (2018). Expressed Sequence Tags (EST). CURRENT APPLIED SCIENCE AND TECHNOLOGY, 273-278.

References

International Human Genome Sequencing Consortium, Nature (London), 409, 2001, 860-921.
J. Kawai, A. Shinagawa, K. Shibata, M. Yoshino, M. Itoh, Y. Ishii, T. Arakawa, A. Hara, Y. Fukunishi and H. Konno, Nature (London), 409, 2001, 685-690.
M.D. Adams, A.R. Kerlavage, C. Fields, and J.C. Venter, Nat. Genet., 4, 1993, 256-267.
M.D. Adams, J.M. Kelley, J.D. Gocayne and M. Dubnick, (1991). Single-run partial sequencing of randomly selected cDNA clones is now a widely used tool in genome research
H. Xiao, C.R. Merril, A. Wu, 6. Olde, R.F. Moreno, A.R. Keravage, W.R. McCombie and J.C. Venter, Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 252, 1991, 1651-1656.

Author Information

Liu Peigang

Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, China.

Yang Qian*

Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, China.

About this Article

Journal

Vol. 4 No. 1 (2004)

Type of Manuscript

Review Article

Keywords

EST, genome, bioinformatics

Published

30 March 2018

Expressed Sequence Tags (EST)

Abstract

How to Cite

References

Author Information

Liu Peigang

Yang Qian*

About this Article

Journal

Type of Manuscript

Keywords

Published

Current Journal

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