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Effects of Ethylthiosulfanylate and Chromium (VI) on the State of Glutathione Antioxidant System and Oxidative Stress Marker Content in Rat Kidneys

Effects of Ethylthiosulfanylate and Chromium (VI) on the State of Glutathione Antioxidant System and Oxidative Stress Marker Content in Rat Kidneys

Original Research ArticleMay 14, 2021Vol. 21 No. 4 (2021)

Abstract

Hexavalent chromium (Cr(VI)) is a heavy metal and powerful toxicant with strong oxidative properties. Antioxidant defense system plays a key role in the processes of elimination and prevention of the negative effects of Cr(VI)-induced oxidative stress in biological systems. Prolonged action of Cr(VI)-induced oxidative stress leads to dysfunction of the antioxidant defense system and as a result provokes cell apoptosis. Ethylthiosulfanylate is synthetic sulfur-containing organic compound that belongs to the class of thiosulfonates. Structurally, thiosulfonates are synthetic analogues of natural organosulfur biologically active substances obtained from garlic, onion, cauliflower and broccoli. Thiosulfonates have antioxidant properties and activate the processes of reactive oxygen species (ROS) utilization. Therefore, the aim of this study was to examine the effect of ethylthiosulfanylate as a synthetic analogue of natural biologically active substances on the state of glutathione antioxidant system and oxidative stress marker content in the kidneys of rats under the condition of Cr(VI)-induced oxidative stress. Our results report that 14 days of ethylthiosulfanylate pretreatment (100 mg/kg body weight) caused attenuation of the intensity of Cr(VI)-induced lipid and protein peroxidation processes (P < 0.05). Moreover, previous impact of ethylthiosulfanylate prevented depletion of the total reduced glutathione (GSH) pool after 14 days of potassium dichromate action in kidneys of rats (P < 0.05). The present study indicates that ethylthiosulfanylate had antioxidant properties and partially inhibited Cr(VI)-induced oxidative damage in kidneys of rats. The obtained results may become a part of the background for the creation of effective methods of prevention and correction of the antioxidant and pro-oxidant states in kidneys affected by the action of Сr(VI)-induced oxidative stress.

Keywords: rats; antioxidant system; kidneys; oxidative stress; ethylthiosulfanylate; hexavalent chromium

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How to Cite

Kotyk*, B. undefined. ., & Iskra, R. undefined. . (2021). Effects of Ethylthiosulfanylate and Chromium (VI) on the State of Glutathione Antioxidant System and Oxidative Stress Marker Content in Rat Kidneys. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 761-773.

References

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Author Information

Bohdan Kotyk*

Department of Biochemistry Adaptation and Ontogenesis of Animals, Institute of Animal Biology of NAAS, Lviv, Ukraine

Ruslana Iskra

Department of Biochemistry Adaptation and Ontogenesis of Animals, Institute of Animal Biology of NAAS, Lviv, Ukraine

About this Article

Journal

Vol. 21 No. 4 (2021)

Type of Manuscript

Original Research Article

Keywords

rats; antioxidant system; kidneys; oxidative stress; ethylthiosulfanylate; hexavalent chromium

Published

14 May 2021