Determination of Hepatitis B Surface Antigen Particles by Elisa

Abstract

Hepatitis B surface Antigen (HBsAg) expressed in Pichia pastoris assemble into particulate structures. Enzyme-linked immunosorbent assay (ELISA) was used to determine these particles. The covalent attachment of enzymes to antibody molecules created an immunological tool possessing both high specificity and high sensitivity. The specificity, rapidity and inexpensive nature of ELISAs have insured their prominence in clinical and veterinary medicine.

Keywords: Hepatits B, Pichia pastoris, ELISA, clinical and veterinary medicine, Antigen

Corresponding author: E-mail: ekwenyeuchechi@yahoo.com

How to Cite

Citation Format

Ekwenye*, U. . (2018). Determination of Hepatitis B Surface Antigen Particles by Elisa. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 44-48.

References

www.nfid.org/factheets/hephadult.html
Boitieux, J.L., Desmet, G. and Thomas, D. 1978 Potentiometric Determination of Hepatitis B Surface Antigen in Biological Fluids, Clinical Chim Acta, 88(2) 329-336.
Frosner, G.G., Schmcrus, H., Wieldmann, K.H., Zachoval, R., Bayerl, B., Backer, U., Gathof, G.A. and Sugg, U. 1982 Diagnostic Significance of Quantitative Determination of Hepatitis B Surface Antigen in Acute and Chronic Hepatitis Infection, European Journal of Clinical Microbiology, 1(1) 52-58.
Chiaramonete, M. Heathcote, J. and Sherlock, S. 1977 Detection by Three Techniques of Hepatisis B Surface Antigen (HBsAg) and Determination of HBsAg and Anti-HBs Titres in Patients with Chronic Liver Disease, Gut, 18, 1-6.
Higgins, D.R and Cregg, J.M. 1998 Pichia Protocols Humana Press. New Jersey, p. 268.

Author Information

U.N. Ekwenye*

Department of Microbiology, Michael Okpara University of Agriculture, Abia State, Nigeria

About this Article

Journal

Vol. 7 No. 1 (2007)

Type of Manuscript

Short Communications

Keywords

Hepatits B, Pichia pastoris, ELISA, clinical and veterinary medicine, Antigen

Published

12 November 2018

Determination of Hepatitis B Surface Antigen Particles by Elisa

Abstract

How to Cite

References

Author Information

U.N. Ekwenye*

About this Article

Journal

Type of Manuscript

Keywords

Published

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