Construction of a Binary Vecter Harboring Bt Gene and Its Transfer to Trichoderma harzianum

Abstract

A binary vertor harboring Bt gene was constructed and was transferred into biocontrol fungus T. harzianum by Agrobacterium tumefaciens-mediated transformation system. Southern blot analysis showed that the Bt gene was integrated into the genome of T. harzianum, and the transformants contained single copies of the gene. The transformation efficiency was 50-160 transformants per 10⁶ spores.

Keywords: Agrobacterium tumefaciens, Bacillus thuringiensis, Trichoderma harzianum, genetic transformation, plasmid construction

Corresponding author: E-mail: cast@kmitl.ac.th

How to Cite

Citation Format

Xingxi, G. ., Qian, Y. ., & Zhaokui, G. . (2018). Construction of a Binary Vecter Harboring Bt Gene and Its Transfer to Trichoderma harzianum. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 69-73.

References

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P.J.C. Cannon, Prospects and progress for Bacillus thuringiensis-based pesticides. Pest. Sci., 37, 1993, 331-335.
M.G. Obukowicz et al., Integration of the deltaendotoxin gene of Bacillus thuringiensis into the chromosome of root colonizing strains of Peseudomonads using Tn5. Gene 45, 1986, 327-331.
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Yan Peisheng et al, Progress of filamentous fungal genetic engineering. Progress of Biological Engineering, 19(1), 1999, 36-41.

Author Information

Gao Xingxi

Department of Life Science and Engineering, Harbin Institute of Technology, China.

Yang Qian

Department of Life Science and Engineering, Harbin Institute of Technology, China.

Guo Zhaokui

Department of Life Science and Engineering, Harbin Institute of Technology, China.

About this Article

Journal

Vol. 4 No. 1 (2004)

Type of Manuscript

Original Research Article

Keywords

Agrobacterium tumefaciens, Bacillus thuringiensis, Trichoderma harzianum, genetic transformation, plasmid construction

Published

30 March 2018

Construction of a Binary Vecter Harboring Bt Gene and Its Transfer to Trichoderma Harzianum

Abstract

How to Cite

References

Author Information

Gao Xingxi

Yang Qian

Guo Zhaokui

About this Article

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Keywords

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