This study aims to seek a new bio-agent as a reducer to synthesize silver nanoparticles (AgNPs). Dried fruiting bodies of truffles were used to mycosynthesize AgNPs, which were described by optical vision, UV-visible spectroscopy, SEM, AFM, Zetasizer, Granularity Cumulation Distribution, and FTIR analyses. The brown color is a sign of mycosynthesizing AgNPs. The UV-visible spectrum exhibited an absorption peak of 450 nm. Images of SEM showed that most of the AgNPs have an average of sizes reached 72 nm with irregular and spherical shapes. The concentration 10 mg/well exhibited 9.5 mm, and 20 mm toward Klebsiella, and P. aeruginosa, respectively. The synergism effect between gentamicin antibiotic and desert truffle-synthesized AgNPs is remarkable to increase the antibacterial activity. The zone of inhibition recorded 18 mm, 18.3 mm, and 20 mm by the conjugation between gentamicin and 5 mg/well of AgNPs compared with 21 mm, 20.6 mm and 23 mm by the conjugation between gentamicin and 10 mg/well against S. aureus, Klebsiella, and P. aeruginosa, respectively.
Keywords: green chemistry; antibacterial activity; Tirmania; mushroom; AgNPs
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