/
/
/
Research and Development of Bioengineering Pesticides, Bacillus thuringiensis WG-001

Research and Development of Bioengineering Pesticides, Bacillus Thuringiensis WG-001

Review ArticleMar 30, 2018Vol. 4 No. 1 (2004)

Abstract

The biopesticides produced by Bacillus thuringiensis have been widely used in the world. On the foundation of exploiting the resource of Bacillus thuringiensis, insecticidal crystal protein genes and helper protein genes were cloned, the appropriate recipient strains were screened, different kinds of vectors were constructed, and expression and regulation of insecticidal crystal protein gene were studies. Environmental safe, highly effective genetically modified Bacillus thuringiensis strains with stable expression have been constructed. Bacillus thuringiensis WG-001 was the first genetically modified pesticide strain which is safety used as a commercial product in China.

Keywords: Bacillus thuringiensis, insecticidal cystal protein genes, genetically modified strains, gene expression and regulation

Corresponding author: E-mail: yz41@mail.hzau.edu.cn

How to Cite

Ziniu*, Y. ., Ming, S. ., Ziduo, L. ., Lin, L. ., & Ying, M. . (2018). Research and Development of Bioengineering Pesticides, Bacillus thuringiensis WG-001. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 156-172.

References

  • Yu Ziniu, Bacillus thuringiensis (Beijing: Science Press, 1990).
  • Schnepf, H.E., and Whiteley, H.R., Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli, Proc. Natl Acad. Sci. USA, 78, 1981, 2893-2897.
  • http://www.biols.susx.ac.uk/home/Neil_Crickmore/B.t/index.html
  • Sun, M, and Yu.Z, Nucleiotide sequence of cry218 in Bacillus thuringiensis YBT-1520, 1997, Accession number AJ002514.
  • Liu Ziduo, Li Lin, Sun Ming, and Yu Ziniu, Cloning of Bacillus thuringiensis subsp. israelensis helper protein genes site-directed mutagenesis by PCR, Journal of Wuhan University (Natural Science Edition), 44, 1998, 13-15.

Author Information

Yu Ziniu*

State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, Huazhong Agriculture University, China.

Sun Ming

State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, Huazhong Agriculture University, China.

Liu Ziduo

State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, Huazhong Agriculture University, China.

Li Lin

State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, Huazhong Agriculture University, China.

Meng Ying

State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, Huazhong Agriculture University, China.

About this Article

Journal

Vol. 4 No. 1 (2004)

Type of Manuscript

Review Article

Keywords

Bacillus thuringiensis, insecticidal cystal protein genes, genetically modified strains, gene expression and regulation

Published

30 March 2018