Production of Cellulase by Trichoderma Viride Under Different Fermentation Conditions

Trichoderma viride has been widely to produce cellulose. The strain studied has the ability to fix nitrogen and produce ethylene that could reach 1.3×10^-6 mol·L^-1·d^-1. In order to utilize this strain better, the production of different cellulolytic enzymes under different fermentation conditions was studied. The results showed that the optimal ratio of rice straw to wheat bran was 3 to 2 or 1 to 1; the optimal nitrogen source was NH₄Cl; the optimal growth temperature was 27-30 °C and the highest production of cellulose enzyme was obtained on the fourth day using solid culture with rice straw and wheat bran. The production of β-glucosidase was very low using liquid medium (Mendel’s), however, the production could be enchanced when 0.2% glucose was added to the culture.

Keywords: cellulose, fixing nitrogen, ferment conditions, β-glucosidase

Corresponding author: E-mail: cast@kmitl.ac.th