Determination of Microcystin LR from Blue-Green Algae (Microcystis aeruginosa Kutz.) Using Reversed-Phase High Performance Liquid Chromatography

Abstract

Microcystin-LR in Microcystis aeruginosa Kutz. collected from Sri Sakhett, Thailand was extracted and quantitatively analyzed using reversed-phase HPLC with UV detection at 238 nm. The HPLC analysis employed a C-18 column (4.6x125 mm., 5 micron), with methanol-water (60 : 40) at flow rate of 1.0 ml/min as the mobile phase. A linear calibration curve was obtained out using five concentrations in the range from 1 to 5 ppm (r² =0.9919). A triplicate analysis of 0.5 g of sample yielded 2.93x10^-⁴ 3.68 x 10^-⁵ g of microcystin-LR (5.85x10^-² 7.35 x 10^-³ % yield). NMR spectroscopy was also used to confirm the structure of the microcystin-LR standard.

Keywords: Microcystis aeruginosa Kutz, reversed-phase HPLC, Microcystin LR, NMR spectroscopic technique

Corresponding author: E-mail: weerachai@mju.ac.th

How to Cite

Citation Format

Phutdhawong*, W. ., Rungrattanakhun, S. ., Sengpracha, W. ., & Suvannachai, N. . (2018). Determination of Microcystin LR from Blue-Green Algae (Microcystis aeruginosa Kutz.) Using Reversed-Phase High Performance Liquid Chromatography. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 522-527.

References

Dow, C.S., Swoboda, U K., 1990. Cyanotoxin. In: Whitton, B.A., Potts, M.(Eds.). The Ecology of Cyanobacteria. Kluwer Academic Publishers, The Netherland, pp. 613-632.
Kaebernick, M., Nielan, B.A., 2001. Ecological and molecular investigations of cyanotoxin production, FEMS Microbiol.Ecol., 35, 1-9.
Briand, J.F., Jacket, S., Bernard, C., Humbert, J.F., 2003. Health hazards for terrestrial vertebrates from toxic cyanobacteria in surface water ecosystems, Vet. Res.,34, 361-377.
Sivonen, K., Jones, G., 1999. Cyanobacteria toxins. In: Chorus, I., Bertram, J. (Eds.). Toxic Cyanobacteria in water. E and F.N. Spon, London, pp. 41-111.
Kuiper-Goodman, T., Falconer, I.R., Figerald, J., 1999. Human health aspects. In: Chorus, I., Bertram, J. (Eds.), Toxic Cyanobacteria in water. E and F.N. Spon, London, pp. 113-153.

Author Information

Weerachai Phutdhawong*

Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.

Siwalee Rungrattanakhun

Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.

Waya Sengpracha

Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon pathom, Thailand.

Napattarapong Suvannachai

Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.

About this Article

Journal

Vol. 6 No. 2b (2006)

Type of Manuscript

Original Research Article

Keywords

Microcystis aeruginosa Kutz, reversed-phase HPLC, Microcystin LR, NMR spectroscopic technique

Published

30 March 2018

Determination of Microcystin LR From Blue-Green Algae (Microcystis Aeruginosa Kutz.) Using Reversed-Phase High Performance Liquid Chromatography

Abstract

How to Cite

References

Author Information

Weerachai Phutdhawong*

Siwalee Rungrattanakhun

Waya Sengpracha

Napattarapong Suvannachai

About this Article

Journal

Type of Manuscript

Keywords

Published

Current Journal

Share

Public URL

Search

Latest Articles

Development of Seasoned Tilapia Fillet with Spicy Stir-fried Curry Paste Product

Physicochemical and Bacteriological Properties of Water from Owo Local Government Area, Nigeria

Formation of Type III Resistant Starch and Alterations in Starch Characteristics and Pasting Behavior of Arrowroot Starch by Autoclaving-Cooling Treatment

Use of Bio-activators in Goat Manure Fermentation and Effects of Solid Goat Manure-based Fertilizer on Growth and Yield of Sweet Corn