cDNA Microarray Technology for the Analysis of Gene Expression

Abstract

DNA Microarray technology is a new and powerful tool for studying a genome-wide gene expression in many orgamism. This technology is a high throughput, highly parallel RNA expression assay technique that permits quantitative analysis of RNAs transcribed from both known and unknown genes. This review describes array manufacturing, experimental design, and its application to gene discovery and expression. Microarray technology id rapidly becoming a central platform for functional genomics.

Keywords: microarray, gene expression, functional genomics

Corresponding author: E-mail: choowonga@hotmail.com

How to Cite

Citation Format

Auesukaree, C. . (2018). cDNA Microarray Technology for the Analysis of Gene Expression. CURRENT APPLIED SCIENCE AND TECHNOLOGY, 29-34.

References

Alwine, J.C., Kemp, D.J. and Stark, G.R. 1977 Method for Detection of Specific RNAs in Agarose Gels by Transfer to Diazobenzyloxymethyl-paper and Hybridization with DNA Probes, Proc Natl Acad Sci U S A, 74(12), 5350-5354.
Liang, P. and Pardee, A.B. 1992 Differential Display of Eukaryotic Messenger RNA by means of the Polymerase Chain Reaction, Science, 257(5072), 967-971.
Schena, M., Shalon, D., Davis, R.W. and Brown, P.O. 1995 Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray, Science, 270(5235), 467-470.
Lipshutz, R.J., Fodor, S.P., Gingeras, T.R. and Lockhart, D.J. 1999 High Density Synthetic Oligonucleotide Arrays, Nat Genet, 21(1 Suppl), 20-24.
Shalon, D., Smith, S.J. and Brown, P.O. 1996 A DNA Microarray System for Analyzing Complex DNA Samples using Two-color Fluorescent Probe Hybridization, Genome Res., 6(7), 639-645.

Author Information

Choowong Auesukaree

Department of Applied Biology, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand

About this Article

Journal

Vol. 6 No. 1 (2006)

Type of Manuscript

Review Article

Keywords

microarray, gene expression, functional genomics

Published

30 March 2018

CDNA Microarray Technology for the Analysis of Gene Expression

Abstract

How to Cite

References

Author Information

Choowong Auesukaree

About this Article

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Type of Manuscript

Keywords

Published

Current Journal

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