Inhibitions of Key Enzymes Relevant to Obesity and Diabetes of Thai Local Mushroom Extracts

The aim of this experiment was to determine inhibitory activities against lipase (anti-obesity property) as well as a-amylase and a-glucosidase (anti-diabetic property) of fifteen Thai local mushroom extracts including Kai-khao (Russula sp.), Kai-kiew (Russula sp.), Nha-lae (Russula sp.), Sa-med (Boletus sp.), E-tun (Russula aff. sp.), Ra-ngok-khao (Amanita aff. sp.), Kor-kar-dang (Russula sp.), Por-nung (Astraeus sp.), Por-fai (Astraeus sp.), Ra-ngok-luang (Amanita sp.), Kor-kar-khao (Russula sp.), Nam-mak (Macowanites sp.), Koh-noi (Russula aff. sp.), Din-khao (Russula sp.) and Khao-pang (Russula sp.) in Amnat-Charoen province, Thailand. All mushrooms were extracted using ddH₂O (20 mg/mL) in a 50°C water bath shaker for 8 hrs. The enzyme inhibitory activities were performed using a colorimetric microplate assay. The results showed that anti-lipase activities of mushroom extracts (4 mg/mL) were in the range of 12-77% inhibition. Din-khao mushroom exhibited the highest inhibition, while Por-fai and Por-nung mushrooms exhibited the lowest. The anti-a-glucosidase activities of mushroom extracts (5 mg/mL) were in the range of 10-79% inhibition. Sa-med mushroom exhibited the highest inhibition, while Kor-kar-dang mushroom exhibited the lowest. However, the inhibition of a-amylase could not be detected in this study. These findings are useful for potential health promotion for Thai local mushroom consumption in controlling obesity and diabetes through key enzymes inhibition.

Keywords: enzyme inhibitory activity, obesity, diabetes mellitus, Thai local mushroom

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 E-mail: uthaiwan.sut@mahidol.ac.th